Single Plate Incubator Shaker

Single Plate Incubator Shaker
Technical Specifications
Accessories
Applications
Technology
Certificates & Manuals

Increase your throughput of automated incubations, without taking valuable space in the workflow. The Inheco Single Plate Incubator Shaker is small, versatile, and fast to reach operating temperature.

Incubator shakers allow you to simultaneously incubate and agitate (or shake) samples of biochemical assays like ELISA or bacterial cells. There’s no need to open the incubator and transfer samples for shaking, the temperature of your samples is maintained throughout the process, and they’re protected from light and contamination. Labs choose Inheco’s single plate incubator shaker with software and verification tool for its versatility and precision.

Easy Integration into robotic workflows

sits on or off the workflow to save space
stack two or more versions together in a stack
adjusted with its own internal controller, enabling automated applications in the workflow
API available, and already integrated with major liquid handling systems

Performance:

simultaneously incubates and shakes samples
temperature of samples is maintained throughout the process and is protected from light and contamination
automatically opening and closing door, enabling automated applications in the workflow
reaches its operating temperature in just 10 minutes
operates up to a maximum temperature of 80 °C with a temperature accuracy of +/-0.3 K and a homogeneity of +/-0.5 K
low vibration shaking of up to 1800 rpm

Features:

shaking amplitude is freely adjustable between 0 - 3mm
free programmable shaking patterns include linear, orbital, elliptic and figure eight
suitable for microplates, deep well plates and vials
pipetting of plates without removing plate from drawer

designed, engineered and made in Germany, rigorous quality control
global two-year warranty and full customer service including remote support, diagnostic and servicing options

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Technical Specifications Incubator Shaker

Incubator type	Single Plate Incubator Shaker MP	Single Plate Incubator Shaker DWP
Input voltage	24 Vdc
Input power	Max 50 W	Max 60 W
Maximum numbers of instruments per power supply (2400121)	3	3
Temperature range	RT +5 K, RT +10 K (for shaking longer than for 1 hour) up to +80 °C (+175 °F)
Tolerable realtive humidity ambient	10-80% relative at +20 °C (+68 °F) up to +30 °C (+86 °F)
Maximum load of the shaker platform	250 g
Shaker frequency range	6.6 Hz to 30 HZ → 400 rpm to 1800 rpm
Maximum shaking amplitude (difference between the maxima)	3 mm
Shaking direction	Arbitrary programmable: e.g. orbital / linear
Dimension in Y_Module w connector cable	295.5 mm
Dimension in Y_Module wo connector cable	268.5 mm
Dimension in X_Module	149 mm
Dimension in Z_Modulewo pin	88,5 mm	139 mm
Open drawer incl. door Y_Drawer	209 mm	239 mm
Dimension of the thermal chamber in ZChamber	18 mm	53 mm
Weigth	4.5 kg	5.4 kg
Maximum Number of instruments in one stack due to interaction during shaking and power limitation of power supply	3	2

Drawer specifications
Accuracy of the end position of the drawer in x, y and z (drawer open); single unit, all parts at specified ambient temperature	±0,30 mm
Maximum drawer load	0,25 kg
Open drawer resistance for a force in Z-axis without plastic deformation	10 N

Environmental conditions
Tolerable relative humidity:
Operation	10-80% relative at +20 °C (+68 °F) up to +30 °C (+86 °F)
Transportation and storage	-10 °C to +60 °C (+14 °F to +140 °F)
Temperature:
Operation	+20 °C to + 30 °C (+68 °F to +86 °F)
Transportation and storage	10 °C to + 60 °C (+14 °F) to 140 °F)

Shaker functionality Incubator Shaker MP / DWP

Parameter

Value

Amplitude range

0.0 - 3.0 mm *

Speed range

6.6 - 30.0 Hz *

Speed precision

±2 Hz

Load

40 - 250 g * (weight of plate and liquid and adapter)

Supervision

speed and amplitude (closed loop controller)

Shaker movement

North - South (linear)
East - West (linear)
Round (orbital)
“Eight” (this is possible but not recommended because it might damage the shaker)

Shaker movement

Amplitude accuracy ±20% (of the target amplitude) Phase shift accuracy ±11 ° ** Validated for the Heating Plate movement / average value over 10 cycles
Single Axis (North - South and East - West) shaker load: 100 g
Shaker	Shaker amplitude [mm]
Speed [Hz]	Single instrument	Maximum stack
10	X = 1.0 to 2.2 or Y = 1.0 to 2.2	X = 1,0 to 1.9 or Y = 1.0 to 1.9
15	X = 1.0 to 2.7 or Y = 1.0 to 2.7	X = 1.0 to 2.4 or Y = 1,0 to 2.4
20	X = 1,0 to 1.2 or Y = 1.0 to 1.2	X = 1.0 to 1.2 or Y= 1.0 to 1.2
Round (phase shift = 90 °)	shaker load: 100 g
Shaker	shaker amplitude [mm]
Speed [Hz]	Single instrument	Maximum stack
10	X = 1.0 to 2.2 Y = 1.0 to 2.2	X = 1,0 to 1.9 Y = 1.0 to 1.9
15	X = 1.0 to 2.7 Y = 1.0 to 2.7	X = 1.0 to 2.4 Y = 1.0 to 2.4
20	X = 1.0 to 1.2 Y = 1.0 to 1.2	X = 1.0 to 1.2 Y = 1.0 to 1.2
„Eight“ (Phase shift = 0 °)	Shaker Load: 100 g
Shaker	Shaker amplitude [mm]
Speed [Hz]	Single instrument	Triple stack
X = 12 / Y = 24	X = 1.0 to 2.4 / Y = 1.0 to 2.4	X = 1.0 to 2.2 / Y = 1.0 to 2.2
X = 15 / Y = 30	X = 1.0 / Y = 1.0	X = 1.0 / Y = 1.0

* For detailed shaker performance, see chapter below.

Thermal functionality for all Incubators

Parameter

Value

Temperature range

Room Temperature plus 5 K to +80 °C [+176 °F]

Accuracy absolute (@ ambient temperature stability ±1K)

The accuracy is defined as the difference between the target temperature of the heating plate and the average of the measured temperature on the heating plate. The heating plate temperature is measured at 5 positions on the heating plate according to the Inheco measurement head PM-0105. The 5 measurement positions are according to the Plate positions A1, H1, E6, A12 and H12.

Target temp. validation tool	Accuracy [°C] for all incubator
+37 °C [+98,6 °F]	± 1.0
+60 °C [+140 °F]	± 1.5
+80 °C [+176 °F] (temperatures higher than +60 °C reduces the uniformity over the labware)	± 2.0

Uniformity measured in well

Uniformity is defined by the maximum difference between lowest and highest temperature according to a Greiner 96 Flat Bottom Plate (Postitions A1, A12, E7, H12)

Target temp. validation tool	Uniformity [°C] for non shaking instruments	Uniformity [°C] for shaking instruments
+37 °C [+98,6 °F]	≤ 0.8	≤ 0.8

Evaporation in unsealed Micro Plates (can be reduced by using lid or cover)

Incubator non shaker temperature +37 °C [+98,6 °F], max. 25% loss of liquid in 4 h, deionized water, room temperature 24-26 °C [75,2-78,8 °F], rel. humidity 40-50%, altitude at 410 m over Sea level	Incubator shaker temperature +37 °C [+98,6 °F], max. 32% loss of liquid in 4 h, deionized water, room temperature 24-26 °C [75,2-78,8 °F], rel. humidity 40-50%, altitude at 410 m over Sea level

Heatup time at start temperature +20 °C [+68 °F] with target temperature +37 °C [+98 °F]

Max. 30 min; Volume 280 µl per well. *	Max.100 min; Volume 1.5 ml per well. **	Max. 30 min; Volume 280 µl per well. *	Max. 120 min; Volume 1.5 ml per well. **

* The target temperature is reached when all sensors are in the tolerance band of 37 °C ±1 °C [98,6 °F ±3,6 °F] Measured with: 1) Greiner 96 Flat Bottom Plate

** The target temperature is reached when all sensors are in the tolerance band of 37 °C ±1 °C [98,6 °F ±3,6 °F] Measured with: 1) NUNC 96 Deep Well Plate

Incubator Shaker Accessories

	IMP Measurement Plate Read more
	Power Supply Nr. 2400121

Why use a single plate incubator shaker?

For some types of cell growth, heat is not enough. Shaking the sample can improve the supply of nutrients as well as oxygen to the cells. While it’s theoretically possible to put a shaker into an incubator, using a combined incubator shaker provides the best conditions for cell growth. It combines heat and movement in one controlled and protected environment.

That makes it invaluable for cell culturing, cell aeration, and solubility studies, for most types of cells including bacterial cultures, tissue cultures, and yeasts.

What to look for in a single plate incubator shaker?
Incubator shakers are available in many different configurations, but temperature ramp rate and accuracy, and choice of shaking patterns are essential to all. Here are some of the main factors to consider.

Size and capacity - with the ability to handle samples in varying cell carriers while fitting comfortably into your workstation. Bigger is not necessarily better for all labs.
Floor-based or bench top - floor-based tend to have larger capacity, bench top may be easier to integrate into your workflow.
Programmability/automation - how will your incubator shaker integrate with your existing set up?
Adjustable speed and shaking patterns - that deliver enough options to meet all your expected requirements.
Temperature range - what is the hottest your assays are likely to require?
Temperature accuracy and uniformity - essential for precise processing of all your assays.
Temperature ramp rate - the faster your cells get to temperature, the higher your throughput can be.
Cleaning and sterilizing - to keep your samples uncontaminated, your incubator shaker should be easy to keep clean.

Use of the Inheco Single Plate Incubator Shaker

The Inheco Single Plate Incubator Shaker is used in NGS-library preparation, PCR, incubation and sequential enzymatic digestions in labs around the world.

It’s often integrated with liquid handling workstations, in combination with a reader and robotic arm.

Labs also use the Inheco Single Plate Incubator Shaker for ELISA, bioassays, NA Hybridization and dark incubation to protect sensitive (fluorescent) dyes from degradation.

Unique shaker drive for more pattern choice

Shaker Drive
The Inheco Incubator Shaker uses a unique shaker drive based on a two-axis pendulum-like swinging system.

As each axis is moved independently, the resulting shaking curve can be controlled by overlaying the x-axis and y-axis movements. That provides a wide choice of shaking curves to be programmed, including linear, circle, elliptic, figure-of-eight and Lissajous figures.

The Inheco Incubator Shaker drive allows the best possible choice of shaking curves with significantly reduced structural vibration of the product.

Direct contact heating with temperature boost for best heating rates

Heating
The Microplate (MP) variants use bottom contact surface heating. Samples heat from 20 °C to 37 °C in approximately 20 minutes.

The DeepWell Plate (DWP) variants heat from all sides within the chamber to avoid condensation during incubation. Samples heat from 20 °C to 37 °C in approximately 10 minutes, up to 4 times faster than in conventional incubators.

Software

API available. The Inheco Single Plate Incubator Shaker is quick to connect, and ready for future developments in your lab.