Single Plate Incubator

Single Plate Incubator
Technical Specifications
Accessories
Applications
Technology
Certificates & Manuals

The Inheco single plate incubator is small, versatile and fast to reach operating temperatures

Incubators provide protected heated environments where your cell samples can grow in optimal conditions, protected from light and contaminants. .

Easy Integration into robotic workflows

sits on or off the workflow to save space
stack two or more versions together in a stack
adjusted with its own internal controller, enabling automated applications in the workflow

Performance:

eaches its operating temperature in just 10 minutes
operates up to a maximum temperature of 80 °C with a temperature accuracy of +/-0.3 K and a uniformity of +/-0.5 K
automatically opening and closing door, enabling automated applications in the workflow

Features:

suitable for microplates, deep well plates and vials
pipetting of plates without removing plate from drawer

designed, engineered and made in Germany, rigorous quality control
global two-year warranty and full customer service including remote support, diagnostic and servicing options

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Technical Specifications Incubator

Incubator type	Single Plate Incubator MP	Single Plate Incubator DWP
Input voltage	24 Vdc
Input power	Max 30 W	Max 45 W
Maximum numbers of instruments per pow-er supply (2400121)	6	4
Temperature range	Room temperature +5 K up to +80 °C (+175 °F)
Tolerable relative humidity ambient	10-80% relative at +20 °C (+68 °F) up to +30 °C (+86 °F)
Maximum load of the shaker platform	N.A.
Shaker frequency range	N.A.
Maximum shaking amplitude (difference between the maxima)	N.A.
Shaking direction	N.A.
Dimension in Y_Module w connector cable	295.5 mm
Dimension in Y_Module wo connector cable	268.5 mm
Dimension in X_Module	149 mm
Dimension in Z_Modulewo pin	58 mm	104 mm
Open drawer incl. door Y_Drawer	193.5 mm	223.5 mm
Dimension of the thermal chamber in ZChamber	18 mm	50 mm
Weight	2.5 kg	3.4 kg
Maximum number of instruments in one stack due to interaction during shaking and power limitation of power supply	5	4

Drawer specifications
Accuracy of the end position of the drawer in x, y and z (drawer open); single unit, all parts at specified ambient temperature	±0,30 mm
Maximum drawer load	0,25 kg
Open drawer resistance for a force in Z-axis without plastic deformation	10 N

Environmental conditions
Tolerable relative humidity:
Operation	10-80% relative at+20 °C (+68 °F) up to +30 °C (+86 °F)
Transportation and storage	-10 °C to +60 °C (+14 °F to +140 °F)
Temperature:
Operation	+20 °C to + 30 °C (+68 °F to +86 °F)
Transportation and storage	-10 °C to + 60 °C (+14 °F) to 140 °F)

Shaker functionality Incubator Shaker MP / DWP

Parameter

Value

Amplitude range

0.0 - 3.0 mm *

Speed range

6.6 - 30.0 Hz *

Speed precision

±2 Hz

Load

40 - 250 g * (weight of plate and liquid and adapter)

Supervision

Speed and amplitude (closed loop controller)

Shaker movement

North - South (linear)
East - West (linear)
Round (orbital)
“Eight” (this is possible but not recommended because it might damage the shaker)

Shaker movement

Amplitude accuracy ±20% (of the target amplitude) Phase shift accuracy ±11 ° ** Validated for the Heating Plate movement / average value over 10 cycles
Single Axis (North - South and East - West) Shaker Load: 100 g
Shaker	Shaker amplitude [mm]
Speed [Hz]	Single instrument	Maximum stack
10	X = 1.0 to 2.2 or Y = 1.0 to 2.2	X = 1,0 to 1.9 or Y = 1.0 to 1.9
15	X = 1.0 to 2.7 or Y = 1.0 to 2.7	X = 1.0 to 2.4 or Y = 1,0 to 2.4
20	X = 1,0 to 1.2 or Y = 1.0 to 1.2	X = 1.0 to 1.2 or Y= 1.0 to 1.2
Round (Phase shift = 90 °)	Shaker load: 100 g
Shaker	Shaker amplitude [mm]
Speed [Hz]	Single instrument	Maximum stack
10	X = 1.0 to 2.2 Y = 1.0 to 2.2	X = 1,0 to 1.9 Y = 1.0 to 1.9
15	X = 1.0 to 2.7 Y = 1.0 to 2.7	X = 1.0 to 2.4 Y = 1.0 to 2.4
20	X = 1.0 to 1.2 Y = 1.0 to 1.2	X = 1.0 to 1.2 Y = 1.0 to 1.2
„Eight“ (Phase shift = 0°)	Shaker load: 100 g
Shaker	Shaker amplitude [mm]
Speed [Hz]	Single instrument	Triple stack
X = 12 / Y = 24	X = 1.0 to 2.4 / Y = 1.0 to 2.4	X = 1.0 to 2.2 / Y = 1.0 to 2.2
X = 15 / Y = 30	X = 1.0 / Y = 1.0	X = 1.0 / Y = 1.0

Thermal functionality for all Incubators

Parameter

Value

Temperature range

Room temperature plus 5 K to +80 °C [+176 °F]

Accuracy absolute (@ ambient temperature stability ±1 K)

The accuracy is defined as the difference between the target temperature of the heating plate and the average of the measured temperature on the heating plate. The heating plate temperature is measured at 5 positions on the heating plate according to the Inheco measurement head PM-0105. The 5 measurement positions are according to the Plate positions A1, H1, E6, A12 and H12.

Target temp. validation tool	Accuracy [°C] for all incubator
+37 °C [+98,6 °F]	±1.0
+60 °C [+140 °F]	±1.5
+80 °C [+176 °F]	±2.0

Uniformity measured in well

Uniformity is defined by the maximum difference between lowest and highest temperature according to a Greiner 96 Flat Bottom Plate (postitions A1, A12, E7, H12)

Target temp. validation tool	Uniformity [°C] for non shaking instruments	Uniformity [°C] for shaking instruments
+37 °C [+98,6 °F]	≤ 0.8	≤ 0.8

Evaporation in unsealed Micro Plates (can be reduced by using lid or cover)

Incubator non shaker temperature +37 °C [+98,6 °F], max. 25% loss of liquid in 4 h, deionized water, room temperature 24-26 °C [75,2-78,8 °F], rel. humidity 40-50%, altitude at 410 m over Sea level	Incubator shaker temperature +37 °C [+98,6 °F], max. 32% loss of liquid in 4 h, deionized water, room temperature 24-26 °C [75,2-78,8 °F], rel. humidity 40-50%, altitude at 410 m over Sea level

Heat-up time at start temperature +20°C [+68°F] with target temperature +37°C [+98°F]

Max. 30 min; Volume 280 µl per well. *	Max. 100 min; Volume 1.5 ml per well. **	Max. 30 min; Volume 280 µl per well. *	Max. 120 min; Volume 1.5 ml per well. **

* The target temperature is reached when all sensors are in the tolerance band of 37 °C ±1°C [98,6 °F ±3,6 °F] Measured with: 1) Greiner 96 Flat Bottom Plate

** The target temperature is reached when all sensors are in the tolerance band of 37 °C ±1°C [98,6 °F ±3,6 °F] Measured with: 1) NUNC 96 Deep Well Plate

Incubator Accessories

	IMP Measurement Plate Read more
	Power Supply Nr. 2400121

Why use a single plate incubator?

Cells need stable and sterile environmental conditions for optimal cell growth. An incubator allows cells to develop in a controlled and protected environment. Due to the compact design and the minimal size of the incubation chamber, single-plate incubators can very quickly compensate for process-related fluctuations in ambient conditions (e.g. when the door is opened or closed). The stress on the cells can thus be minimized.

What to look for in a single plate incubator
Incubators are available in many different configurations, but temperature ramp rate and accuracy plus protection from light and contamination are essential to all. Here are some of the main factors to consider.

Size and capacity - with the ability to handle samples in varying cell carriers while fitting comfortably into your workstation. Bigger is not necessarily better for all labs.
Floor-based or bench top - floor-based tend to have larger capacity, bench top may be easier to integrate into your workflow.
Programmability/automation - how will your incubator integrate with your existing set up?
Temperature range - what is the hottest your assays are likely to require?
Temperature accuracy and uniformity - essential for precise processing of all your assays.
Temperature ramp rate - the faster your cells get to temperature, the higher your throughput can be.
Cleaning and sterilizing - to keep your samples uncontaminated, your incubator should be easy to keep clean.

Use of the Inheco Single Plate Incubator

The Inheco Single Plate Incubator is used in NGS-library preparation, PCR, incubation and sequential enzymatic digestions in labs around the world.

It’s often integrated with liquid handling workstations, in combination with a reader and robotic arm.

Labs also use the Inheco Single Plate Incubator for ELISA, bioassays, NA Hybridization and dark incubation to protect sensitive (fluorescent) dyes from degradation.

Direct contact heating with temperature boost for best heating rates

Heating
The Microplate (MP) variants use bottom contact surface heating. Samples heat from 20 °C to 37 °C in approximately 20 minutes.

The Deep Well Plate (DWP) variants heat from all sides within the chamber to avoid condensation during incubation. Samples heat from 20 °C to 37 °C in approximately 10 minutes, up to 4 times faster than in conventional incubators.

Software

API available. The Inheco Single Plate Incubator is quick to connect, and ready for future developments in your lab.